Molecular Cloning of Canine Membrane-Anchored Inhibitor of Matrix Metalloproteinase, RECK

Takagi, Satoshi and Kitamura, Takanori and Hosaka, Yoshinao and Ohsaki, Tomohiro and Bosnakovski, Darko and Kadosawa, Tsuyoshi and Okumura, Masahiro and Fujinaga, Toru (2005) Molecular Cloning of Canine Membrane-Anchored Inhibitor of Matrix Metalloproteinase, RECK. Journal of Veterinary Medical Science, 67. pp. 385-391.

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Abstract

The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) gene is one of the endogenous matrix metalloproteinase (MMP) inhibitors. It was reported that decreased RECK expression closely correlated with tumor malignancy. We determined the cDNA sequence of the canine RECK gene. The cDNA sequence and deduced amino acid of canine RECK were 2,913 bases and 971 residues, respectively. The predicted amino acid sequence of the protein showed 95.5% and 91.9% homology with human and mouse RECK, respectively. RECK mRNA expression was analyzed in various canine tissues and tumor cell lines by quantitative RT-PCR. The highest RECK expression was detected in lung and testis. In comparison with the tissues, a remarkably low expression level was detected in tumor cell lines. In addition, the RECK gene was transfected in the canine transitional cell carcinoma, and its influence on cell proliferation, migration, and invasion was analyzed. The transfected RECK gene suppressed only canine tumor invasion. These results showed that RECK might play an important role in tumor malignancy in dogs as well as in other mammalians.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Darko Bosnakovski
Date Deposited: 30 Nov 2012 14:00
Last Modified: 30 Nov 2012 14:00
URI: http://eprints.ugd.edu.mk/id/eprint/2533

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