l-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent peroxidation in rat's liver and kidney

Hadzi-Petrusev, Nikola and Jankulovski, Nikola and Milev, Mishko and Filipovska, Pavlina and Gagov, Hristo and Gjorgievska, Elizabeta and Mitrov, Dine and Sopi, Ramadan and Hristov, Kiril and Mladenov, Mitko (2012) l-2-oxothiazolidine-4-carboxylate influence on age- and heat exposure-dependent peroxidation in rat's liver and kidney. Journal of Thermal Biology, 37 (5). pp. 361-365.

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Abstract

Abstract To investigate the impact of acute heat exposure on maintenance of redox homeostasis and antioxidant balance related to aging, we have determined the GSH levels in the liver and kidney, and the activity of antioxidant enzymes in the same organs from Wistar rats at two different ages, 35 days and 18 months. The animals were housed individually in a special heated chamber maintaining a constant temperature of 40±0.5 °C. The results showed that the level of endogenous GSH was significantly lower in aged than in young animals. In general, the activity of antioxidant enzymes in investigated tissues displayed an age-dependent decline. Indeed, we found unchanged CAT activity and decreased GPx activity with age. On the other hand acute heat exposure led to disproportion between peroxide metabolizing enzymes (CAT, GPx) and GR, thus promoting H2O2 accumulation and prooxidative state in the liver of young animals. The results for the impact of l-2-oxothiazolidine-4-carboxylate in combined stress model suggested that in spite of restore levels of GSH, the restoration of oxido-reductive balance might have only been partial due to irreversible alterations in antioxidant enzymes set by acute heat exposure and aging. Interestingly, young animals appeared to be more sensitive to the supplementation of the l-2-oxothiazolidine-4-carboxylate, likely because of the more extensive increase of GSH observed in young l-2-oxothiazolidine-4-carboxylate treated animals.

Item Type: Article
Uncontrolled Keywords: Antioxidant enzymes Aging Heat exposure Wistar rats Glutathione
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Misko Milev
Date Deposited: 01 Nov 2012 09:09
Last Modified: 15 Aug 2013 13:50
URI: http://eprints.ugd.edu.mk/id/eprint/251

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