Phenotypic and pathogenic characterization of Colletotrichum spp. associated with bitter rot on apple fruits in post-harvest storage

Kovacevik, Biljana and Mitrev, Sasa (2019) Phenotypic and pathogenic characterization of Colletotrichum spp. associated with bitter rot on apple fruits in post-harvest storage. In: 2nd International Meeting Agriscience & Practice, 12 Apr 2019, Stip.

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Abstract

Postharvest diseases are major problem in long storage of apple (Malus pumila Mill.) worldwide. Among them, bitter rot caused by Colletotrichum spp. complex (C. gleosporioides and C. accutatum) is one of the prevalent. The pathogen affects the fruit pre-harvest in orchards and/or post-harvest in storage, resulting in considerable economic losses. Advanced symptoms of bitter rot and anthracnose canker, with dark ‐brown to black fruiting bodies called acervuli, were visible on stored apple fruits grown in the region of Berovo, Republic of Macedonia. Acervuli bear abundant one-celled fusiform macroconidia. Two groups of fungal isolates were obtained using standard laboratory procedure on PDA medium. The first group formed white mycelium that become smoke-grey with the time forming black aggregated conidiomata. The second group formed white areal mycelium with orange-pink colour on the reverse side with orange-pink conidiomta that appear black on the reverse side. One-celled elliptic to fusiform macroconidia are observed on PDA medium. Microconidia were not observed. The pathogenicity of the isolates was tested by wound inoculation of healthy 'Idared' apple fruit. After ten days, post-inoculation lesions up to 2-3 cm in diameter were developed on inoculated fruit while the control remained healthy. Acervuli was also developed in the rotted areas of inoculated fruits. Biological and morphological characterization of isolates was performed in vitro. The isolates were preliminary identified based on the morphology, temperature of growth and sensitivity of pesticides. Due to the overlapping morphological characters, species delimitation based on morphology alone is hardly possible in the genus of Colletotrichum. Multilocus sequence analyses combined with a polyphasic approach is generally suggested for species differentiation.

Item Type: Conference or Workshop Item (Poster)
Subjects: Agricultural Sciences > Other agricultural sciences
Divisions: Faculty of Agriculture
Depositing User: Biljana Kovacevic
Date Deposited: 24 Sep 2019 07:47
Last Modified: 24 Sep 2019 07:47
URI: http://eprints.ugd.edu.mk/id/eprint/22554

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