Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods

Bogeski, Ivan and Mirceski, Valentin and Hoth, Markus (2008) Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods. Clinical Chemical Laboratory Medicine, 46 (2). ISSN 14346621

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Official URL: http://dx.doi.org/10.1515/CCLM.2008.023

Abstract

Background: Reactive oxygen species and redox signaling play an important role in the regulation of many vital biological processes. However, they are also tightly connected with many pathological conditions. The detection and evaluation of these signaling events are very often accompanied with great difficulties. In this article, we describe the development of a novel electrochemically-based technique for monitoring the cellular redox state. Methods and results: T-cells were attached on the surface of a working electrode, which was modified with 2-palmitoylhydroquinone as a redox mediator. Using cyclic voltammetry, we were able to indirectly (via the redox mediator) monitor an electron transport from the cells towards the working electrode, which enabled us to evaluate the redox activity of the cells. Conclusions: This new technique is rather simple and sensitive and may be used in the future as a valid diagnostic procedure in various branches of biomedical science.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Chemical sciences
Divisions: Faculty of Medical Science
Depositing User: Ivan Bogeski
Date Deposited: 27 Nov 2012 10:43
Last Modified: 27 Nov 2012 10:43
URI: http://eprints.ugd.edu.mk/id/eprint/2198

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