Can We See PIP3and Hydrogen Peroxide with a Single Probe?

Mishina, Natalie M. and Bogeski, Ivan and Bolotin, Dmitry A. and Hoth, Markus and Niemeyer, Barbara A. and Schultz, Carsten and Zagaynova, Eleva V. and Lukyanov, Sergey and Belousov, Vsevolod V. (2012) Can We See PIP3and Hydrogen Peroxide with a Single Probe? Antioxidants & Redox Signaling, 17 (3). pp. 505-512. ISSN 1523-0864

[img] Text
ARS-2012.pdf

Download (808Kb)
Official URL: http://dx.doi.org/10.1089/ars.2012.4574

Abstract

A genetically encoded sensor for parallel measurements of phosphatidylinositol 3-kinase activity and hydrogen peroxide (H2O2) levels (termed PIP-SHOW) was developed. Upon elevation of local phosphatidylinositol 3,4,5- trisphosphate (PIP3) concentration, the sensor translocates from the cytosol to the plasma membrane, while a ratiometric excitation change rapidly and simultaneously reports changes in the concentration of H2O2. The dynamics of PIP3 and H2O2 generation were monitored in platelet-derived growth factor-stimulated fibroblasts and in T-lymphocytes after formation of an immunological synapse. We suggest that PIP-SHOW can serve as a prototype for many fluorescent sensors with combined readouts.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Health sciences
Divisions: Faculty of Medical Science
Depositing User: Ivan Bogeski
Date Deposited: 27 Nov 2012 10:42
Last Modified: 27 Nov 2012 10:42
URI: http://eprints.ugd.edu.mk/id/eprint/2195

Actions (login required)

View Item View Item