Vibrational Spectroscopy as a Tool for Examination to the Secondary Structure of Metal-labeled Trastuzumab Immunoconjugates

Sterjova, Marija and Dzodic, Predrag and Makreski, Petre and Duatti, Adriano and Risteski, Milan and Janevik-Ivanovska, Emilija (2019) Vibrational Spectroscopy as a Tool for Examination to the Secondary Structure of Metal-labeled Trastuzumab Immunoconjugates. Journal of Radioanalytical and Nuclear Chemistry, 320 (1). pp. 209-218. ISSN 1588-2780

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Official URL: https://link.springer.com/article/10.1007/s10967-0...

Abstract

Infrared and Raman spectroscopy are effective techniques that allow collecting information about secondary structure of proteins, including antibodies. Trastuzumab, antibody used in our study was in a freeze-dried form, conjugated with different bifunctional chelators and linked with the stable isotopes of lutetium and yttrium. The characterization of the final immunoconjugates showed no significant changes in the structure demonstrated by the presence of the amide bands characteristic for a α-helices and β-sheets structures. These methods could be applied during the production of the antibody freeze-dried kit formulations for the labeling with the radioactive isotopes.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Divisions: Faculty of Medical Science
Depositing User: Marija Arev
Date Deposited: 28 Mar 2019 09:09
Last Modified: 28 Mar 2019 09:09
URI: http://eprints.ugd.edu.mk/id/eprint/21835

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