New Aspects of Protein-film Voltammetry of Redox Enzymes Coupled to Follow-up Reversible Chemical Reaction in Square-wave Voltammetry

Gulaboski, Rubin and Janeva, Milkica and Maksimova, Viktorija (2019) New Aspects of Protein-film Voltammetry of Redox Enzymes Coupled to Follow-up Reversible Chemical Reaction in Square-wave Voltammetry. Electroanalysis, 31 (2). pp. 1-12. ISSN 10400397

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Official URL: http://dx.doi.org/10.1002/elan.201900028

Abstract

Protein-film square-wave voltammetry of uniformly adsorbed molecules of redox lipophilic enzymes is applied to study their electrochemical properties, when a reversible follow-up chemical reaction is coupled to the electrochemically generated product of enzyme’s electrode reaction. Theoretical consideration of this so-called “surface ECrev mechanism” under conditions of squarewave voltammetry has revealed several new aspects, especially by enzymatic electrode reactions featuring fast electron transfer. We show that the rate of chemical removal/resupply of electrochemically generated Red (ads) enzymatic species, shows quite specific features to all current components of calculated square-wave voltammograms and affects the electrode kinetics. The effects observed are specific for this particular redox mechanism (surface ECrev mechanism), and they got more pronounced at high electrode kinetics of enzymatic reaction. The features of phenomena of “split net-SWV peak” and “quasireversible maximum”, which are typical for surface redox reactions studied in square-wave voltammetry, are strongly affected by kinetics and thermodynamics of follow-up chemical reaction. While we present plenty of relevant voltammetric situations useful for recognizing this particular mechanism in square-wave voltammetry, we also propose a new approach to get access to kinetics and thermodynamics of follow-up chemical reaction. Most of the results in this work throw new insight into the features of protein-film systems that are coupled with chemical reactions.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Natural sciences > Matematics
Natural sciences > Physical sciences
Divisions: Faculty of Medical Science
Depositing User: Rubin Gulaboski
Date Deposited: 21 Feb 2019 11:18
Last Modified: 21 Feb 2019 11:18
URI: http://eprints.ugd.edu.mk/id/eprint/21585

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