Time-Independent Methodology to Access Michaelis-Menten Constant by Exploring Electrochemical-Catalytic Mechanism in Protein-Film Cyclic Staircase Voltammetry

Gulaboski, Rubin and Kokoskarova, Pavlinka and Petkovska, Sofija (2018) Time-Independent Methodology to Access Michaelis-Menten Constant by Exploring Electrochemical-Catalytic Mechanism in Protein-Film Cyclic Staircase Voltammetry. Croatica Chemica Acta, 91 (3). ISSN 00111643

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Official URL: http://dx.doi.org/10.5562/cca3383

Abstract

Protein-film voltammetry is recognized as a very efficient tool in mechanistic enzymology, but it is also seen as a relevant approach to gain thermodynamic and kinetic information related to the redox chemistry of many enzymes. This technique requires a small amount of redox enzyme, whose molecules form monomolecular film on the working electrode surface. In this paper we present a simple and time-independent cyclo-voltammetric method for the determination of kinetics of the chemical step of an electrochemical-catalytic (EC’) mechanism in protein-film scenario. Theoretical results of a surface EC’ mechanism show that the limiting cyclo-voltammetric catalytic current, measured at large overpotentials, depends solely on the rate of the chemical regenerative reaction. At large overpotentials, the limiting current of the steady-state cyclic voltammograms is independent on all kinetics and thermodynamic parameters related to the electrode reaction of adsorbed enzyme. The approach proposed relies on the dependence of the magnitude of limiting current of the experimental cyclic steady-state voltammograms as a function of the substrate concentration.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Chemical sciences
Natural sciences > Matematics
Natural sciences > Physical sciences
Divisions: Faculty of Medical Science
Depositing User: Rubin Gulaboski
Date Deposited: 13 Dec 2018 11:36
Last Modified: 13 Dec 2018 11:37
URI: http://eprints.ugd.edu.mk/id/eprint/20976

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