Total antioxidants in plasma of hemodialysed patients and healthy controls measured by two commercial assays

Ruskovska, Tatjana and Jansen, Eugene (2012) Total antioxidants in plasma of hemodialysed patients and healthy controls measured by two commercial assays. In: 20th Meeting of Balkan Clinical Laboratory Federation, 19 – 22 September 2012, Belgrade, Serbia.

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Abstract

Introduction: As a result of an increased interest in oxidative stress research, in both basic and clinical studies, numerous commercial test kits became available. The aim of this study was to evaluate the results for total antioxidants measured by two commercial assays in a complex clinical condition such as single hemodialysis session in patients on chronic hemodialysis treatment, in comparison to healthy controls. Methods: The level of plasma total antioxidants was measured by BAP (Biological Antioxidant Potential; Diacron, Grosseto, Italy) and TAS (Total Antioxidant Status; Rel Assay Diagnostics, Gaziantep, Turkey) commercial test kits. 32 patients, adults with end-stage renal disease, on chronic hemodialysis treatment for more than 1 year, and treated with a protocol of three hemodialysis sessions a week, were included in the study. The control group consisted of 47 volunteers selected from healthy candidates for military service. Results: Patients before hemodialysis had significantly higher BAP values compared to the healthy controls (p<0,01). As a result of the hemodialysis session the average BAP value dropped with 12,6% (p<0,001 when compared to the values before hemodialysis). The BAP values measured after the hemodialysis were significantly lower than those of healthy controls (p<0,01). When measured by the TAS assay, patients before hemodialysis again had higher values of total antioxidants than controls, but the difference showed higher statistic significance: p<0,001. As a result of the hemodialysis session the average TAS value decreased more (35,2%) than that of BAP (p<0,001 when compared to the values before hemodialysis). The TAS values measured after the hemodialysis were lower than those measured in healthy controls, but this difference was not statistically significant. Discussion: From our results it is clear that for the same set of samples tested in parallel by two commercial methods for measurement of total antioxidants sometimes different conclusions can be drawn.

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Health sciences
Divisions: Faculty of Medical Science
Depositing User: Tatjana Ruskovska
Date Deposited: 24 Nov 2012 13:11
Last Modified: 24 Nov 2012 13:11
URI: http://eprints.ugd.edu.mk/id/eprint/1892

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