Genotoxicity tests and their contributions in ecotoxicological research

Velickova, Nevenka and Milev, Mishko (2016) Genotoxicity tests and their contributions in ecotoxicological research. In: 5th Congress of Ecologists of the Republic of Macedonia with international participation, 19-22 Oct 2016, Ohrid, Republic of Macedonia.

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Abstract

Introduction: The cytokinesis-block micronucleus cytome assay (CBMN) is suitable and standardized test in genotoxicology used for assessment of genotoxicological effects of different chemical or physical agents and it is the most commonly used method for measuring DNA damage in human lymphocytes. The micronucleus assay, like basic genotoxic test in molecular epidemiology and cytogenetics, is applied as diagnostic tool and procedure for measuring micronucleus (MN) frequency in peripheral blood lymphocytes (PBL). The aim of this study was to evaluate the genotoxicity of ionizing radiation using the CBMN assay and to determine the human health risk. Material and methods: The study population included 20 healthy workers exposed to ionizing radiation (radiologist, technicians and nurses) and 20 individuals healthy people not exposed on radiation. Results: 12 individual samples (60 %) showed an increase in the MN frequency while in the control group, the increased frequency of MNi was found in 1 of the blood samples (5%). Conclusion: These results suggest that chromosomal instability is in the correlation with micronucleus frequencies and genetic load in healthy workers. This study has a practical importance because it indicates the necessity of introducing a permanent genotoxicological and other monitoring on the vulnerable category of workers (in this study medical workers).

Item Type: Conference or Workshop Item (Poster)
Subjects: Natural sciences > Biological sciences
Divisions: Faculty of Medical Science
Depositing User: Nevenka Velickova
Date Deposited: 28 Nov 2016 11:03
Last Modified: 28 Nov 2016 11:03
URI: http://eprints.ugd.edu.mk/id/eprint/16698

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