Comparative determination of biogenic amines by HPLC-DAD and UPLC-TQ/MS techniques: advantages and disadvantages

Tasev, Krste and Ivanova, Violeta and Stefova, Marina (2014) Comparative determination of biogenic amines by HPLC-DAD and UPLC-TQ/MS techniques: advantages and disadvantages. In: 24 Congress of Chemists and Technologists of Macedonia, 11-14 Sep 2016, Ohrid, Macedonia.


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Biogenic amines are organic nitrogen compounds with low molecular weight whose presence plays a vital role in oenology. They can be found in the grape must, but they can also be formed by yeast during alcoholic and malolactic fermentation, as well as during wine aging. Their presence in wine at high levels can lead to health problems. The most important biogenic amines in wine are tryptamine (TRP), putrescine (PUT), histamine (HIST), phenylethylamine (PEA), tyramine (TYR), cadaverine (CAD), spermine (SPM) and spermidine (SPD). The mostly used technique for determination of biogenic amines in wine is pre-column derivatization with dansyl chloride (DnsCl) and reversed phase-high performance liquid chromatography with diode array detector (RP-HPLC-DAD) [1]. Ultra-performance liquid chromatography (UPLC) coupled with triple quadruple detector mass spectrometer (TQ/MS) is recently introduced technique for biogenic amines analysis. The aim of this study was to compare the performances and critical validation points of two validated methods (HPLC-DAD and UPLC-TQ/MS), such as linearity, recovery, repeatability and reproducibility in order to point out their advantages and disadvantages and to choose right method for the laboratory purposes. It was found that the main advantages of the UPLC-TQ/MS method are direct injection of the wine samples without previous sample preparation with total run time of 5 min and lower LOQ. Thus, the LOQ for histamine determined with UPLC-TQ/MS and RP-HPLC-DAD was 1.50 µg/L and 20 µg/L, respectively. On the other hand, 45 min derivatization of biogenic amines is required for sample preparation and 30 min analyses run time for the RP-HPLC-DAD. In general, both methods are characterized with good linearity (R2 > 0.9950), satisfactory recovery, repeatability and reproducibility.

Item Type: Conference or Workshop Item (Poster)
Subjects: Natural sciences > Chemical sciences
Divisions: Faculty of Agriculture
Depositing User: Violeta Ivanova Petropulos
Date Deposited: 27 Oct 2016 10:18
Last Modified: 27 Oct 2016 10:18

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