Enzymatic formation of ions and their detection at a three-phase electrode

Gulaboski, Rubin and Pereira, Carlos M. and Cordeiro, M Natália D S and Bogeski, Ivan and Silva, A. Fernando (2005) Enzymatic formation of ions and their detection at a three-phase electrode. Journal of Solid State Electrochemistry, 9 (6). pp. 469-474. ISSN 1432-8488

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Official URL: http://dx.doi.org/10.1007/s10008-005-0696-0


An electrochemical method for the detection of enzymatically created anions is described that uses a thin-film electrode with decamethylferrocene as an electroactive redox probe. The enzymatic oxidation of glucose with enzyme glucose oxidase produces gluconic acid as a final product. The oxidation of decamethylferrocene dissolved in the thin-nitrobenzene film, that is spread on the working graphite electrode and submerged in the aqueous solution containing glucose and glucose oxidase, is followed by the up-take of gluconate anions from the aqueous phase to nitrobenzene. The peak currents of the square-wave voltammetric responses of that system are a linear function of the glucose concentration in the milimolar range from 0.1 mmol/L to 0.7 mmol/L (R2=0.994).

Item Type: Article
Uncontrolled Keywords: glucose, electrochemical sensor, ion transfer across biomimetic membranes
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Natural sciences > Physical sciences
Divisions: Faculty of Agriculture
Depositing User: Rubin Gulaboski
Date Deposited: 31 Oct 2012 13:14
Last Modified: 05 Nov 2012 13:23
URI: http://eprints.ugd.edu.mk/id/eprint/155

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