HPLC/DAD Determination of Aloin Besides Flavonoids in Complex Plant Pharmaceuticals

Shishovska, Maja and Arsova-Sarafinovska, Zorica and Doneva, Dragica and Ameti, Agim and Poposka-Svirkova , Zaklina and Starkoska, Katerina (2014) HPLC/DAD Determination of Aloin Besides Flavonoids in Complex Plant Pharmaceuticals. In: XXIII Congress of Chemists and Technologists of Macedonia, 8-11 Oct 2014, Ohrid, Macedonia.

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Programe of XXIII Congress Of Chemists and Technologists of Macedonia 2014_Ohrid.pdf

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HPLC_DAD Determination of Aloin Besides Flavonoids in Complex Plant Pharmaceuticals.pdf

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Official URL: http://www.pmf.ukim.edu.mk/PMF/Chemistry/sojuz/23c...

Abstract

A simple HPLC/DAD method for determination of aloin in complex matrices is proposed, using aloin A as a marker compound. The chromatographic separation was achieved on C18 HPLC column, mobile phase composed of acetonitrile and water (pH 2.6) in gradient mode, flow-rate 1.5 ml/min, with simultaneous UV detection at 295 nm for aloin, and 255 nm and 375 nm for flavonoids. For the method the obtained validation results are in satisfactory ranges. This method is successfully applied for analysis of aloin in Aloe barbadensis Mill. plant dried exudate and pharmaceuticals which besides this exudate contain mixture of different extracts from: Achillea Millefolium L., herba; Calendula officinalis L., flos; Cornux mas L., plant cortex; Cynodon dactylon L., rhizome; Hypericum perforatum L., herba; Inula helenium L., radix and Viscum album L., herba. It is shown that other present compounds such as flavonoids (quercetin, hyperozid and rutin) do not interfere in determination and they may be analyzed simultaneously. This method is proposed to be used in control of pharmaceuticals.

Item Type: Conference or Workshop Item (Poster)
Subjects: Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Zorica Arsova Sarafinovska
Date Deposited: 23 Jun 2015 08:48
Last Modified: 23 Jun 2015 08:48
URI: http://eprints.ugd.edu.mk/id/eprint/13404

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