Performance evaluation of different HPLC columns in sildenafil and tadanafil analysis

Poposka, Zaklina and Shishovska, Maja and Arsova-Sarafinovska, Zorica and Doneva, Dragica and Starkoska, Katerina and Mustafa, Zana (2011) Performance evaluation of different HPLC columns in sildenafil and tadanafil analysis. European Journal of Pharmaceutical Sciences, 44. pp. 74-75. ISSN 1879-0720

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Official URL: http://www.sciencedirect.com/science/journal/09280...

Abstract

Sildenafil and tadalafil are oral drugs used to treat male sexual function problems (impotence or erectile dysfunction) by blocking an enzyme 5-phosphodiesterase in the body. The aim of this study was to evaluate performance of the various HPLC columns in sildenafil and tadalafil analysis using validated HPLC method. The following columns were compared: LiChrospher 100 RP-18 (250 x 4 mm i.d., 5 m); Hypersil BDS-C18 (125 x 4 mm i.d., 5 m) and Chromolith Performance RP-18e (100 x 4.6 mm i.d., monolithic rod). The performance evaluation was done by comparison of the following parameters: resolution (Rs), back-pressure (ΔP, bar), and theoretical plate height (ΔH, m) in correlation with flow-rate (u, mL/min). Chromatographic peak resolution data (Rs) obtained are acceptable for all three tested columns with values higher than the limit given in Ph. Eur. (>1.5). There is a significant difference between column back-pressure using different flow-rate. The efficiency of the columns is presented by the van Deemter plots. According to the results, the most efficient column is the longest column packed with particles, but the flow-rate which might be used is limited at maximum 2 ml/min. With increasing flow-rate its efficiency dramatically decreases. The van Deemter plot of the monolithic rod column demonstrates clearly that separation efficiency does not decrease significantly when the flow-rate is increased, as it is the case with particulate columns. According to all experimental results obtained, the monolithic rod column is a column of choice for tadalafil and sildenafil analysis. Using this column means shorter analysis time (for factor 3.6) in comparison with the longest column.

Item Type: Article
Subjects: Natural sciences > Chemical sciences
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Zorica Arsova Sarafinovska
Date Deposited: 10 Jun 2015 12:10
Last Modified: 10 Jun 2015 12:10
URI: http://eprints.ugd.edu.mk/id/eprint/13263

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