A Simple Isocratic RP-HPLC Method for Quality Control of Oseltamivir Capsules

Ameti, Agim and Slavkovska, Jasmina and Starkoska, Katerina and Arsova-Sarafinovska, Zorica (2012) A Simple Isocratic RP-HPLC Method for Quality Control of Oseltamivir Capsules. Macedonian Journal of Chemistry and Chemical Engineering, 31 (2). pp. 205-215. ISSN 1857-5552

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Abstract

A simple isocratic reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for determination of oseltamivir active pharmaceutical ingredient (API) in bulk drug and pharmaceuticals. The separation was achieved on a Purospher STAR® RP – 18e column with a mobile phase consisting of methanol - 0.02 mol l–1 phosphate buffer, pH 5, 50:50 (V/V). Chromatographic results demonstrated the specificity of the method for determination of oseltamivir in presence of degradation products generated in studies of forced decomposition. The limit of detection (LOD) and limit of quantification (LOQ) for oseltamivir phosphate were 0.0162 μg ml–1 and 0.0491 μg ml–1, respectively. The advantages of this method include simple sample treatment, short elution time (less than 6 min) and short analysis time (less than 10 min). Furthermore, using methanol instead of acetonitrile in a mobile phase composition considerably reduces the laboratory expenses, still retaining adequate sensitivity for routine analysis as well as for evaluation of authenticity of Tamiflu® products.

Item Type: Article
Uncontrolled Keywords: oseltamivir, RP-HPLC, HPLC-DAD, quality control, pharmaceuticals
Subjects: Engineering and Technology > Chemical engineering
Natural sciences > Chemical sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Zorica Arsova Sarafinovska
Date Deposited: 08 May 2015 11:23
Last Modified: 08 May 2015 11:25
URI: http://eprints.ugd.edu.mk/id/eprint/13141

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