Lactobacillus casei Loaded Alginate-Soy Protein Microparticles: acidification Kinetics and Survival of the Probiotic in Simulated Gastrointestinal Conditions

Hadzieva, Jasmina and Stefanovska, Ana and Petrusevska Tozi, Lidija and Rafajlovska, Vesna and Petreska Ivanovska, Tanja and Dodov Glavas, Marija and Crcarevska Simonovska, Maja and Pavlova-Jurhar, Maja and Smilkov, Katarina and Mladenovska, Kristina (2014) Lactobacillus casei Loaded Alginate-Soy Protein Microparticles: acidification Kinetics and Survival of the Probiotic in Simulated Gastrointestinal Conditions. In: VI Serbian Congres of Pharmacy with international participations, 15-19 Oct 2014, Belgrade, Serbia.

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Abstract

L. casei has already proven its health effects. However, its viability decreases after exposure to gastric juice and bile salts. The aim of this study was to protect the probiotic microorganism from the harsh environment of the GIT by microencapsulation in alginate-soy microparticles. Aqueous dispersion of alginate (2.5%w/w) and probiotic cells (ca12log CFU/g) was emulsified in olive oil containing 0.2% Tween 80 to obtain microparticles which were subsequently cross-linked (CaCl2, 3%w/w), coated with the protein (1:4-4:1 in respect to alginate), isolated, washed and stored (0.9% saline, 4oC). Negatively charged microparticles were obtained (d50 16-36um, Ca-content 5.56-9.38%), viability 9.11-11.25log CFU/g). Free and encapsulated cells were cultivated in MRS broth (37oC) to determine if they were still metabolically active. pH values and optical density at 600nm were measured every 4h. Viability tests of free and encapsulated cells were performed by exchanging simulated GI juices, after incubation. The enumeration of living cells was assayed by incubation on MRS agar (37oC, 48h). The time taken to decrease the initial pH of MRS broth to 4 was 20h for free cells and for encapsulated cells 32-56h. Initial decrease in cell survival was observed after 0.5h (70% for free, 20-35% for encapsulated cells). After 12 h, the viability of the encapsulate cells was 5.7-8.6 log CFU/g. In conclusion, encapsulated L. casei in alginate-soy protein microparticles showed significantly higher survival in simulated GIT compared to free cells. The use of protein increased the survival compared to alginate alone.

Item Type: Conference or Workshop Item (Poster)
Subjects: Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Medical and Health Sciences > Health biotechnology
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Katarina Smilkov
Date Deposited: 01 Apr 2015 11:30
Last Modified: 01 Apr 2015 11:30
URI: http://eprints.ugd.edu.mk/id/eprint/12975

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