Anti-inflammatory properties of L. casei loaded whey protein-alginate microparticles in animal model of colitis

Smilkov, Katarina and Dodov Glavas, Marija and Hadzieva, Jasmina and Pavlova Jurhar, Maja and Petreska Ivanovska, Tanja and Ristoski, Trpe and Gjurovski, Ivica and Petrusevska Tozi, Lidija and Mladenovska, Kristina (2014) Anti-inflammatory properties of L. casei loaded whey protein-alginate microparticles in animal model of colitis. In: 9th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical technology, 31 March - 3 Apr 2014, Lisbon, Portugal.

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Inflammatory bowel diseases are chronic conditions that affect large population and the drugs used for their treatment have great potential for manifesting adverse effects. Regular administration of probiotics incorporated in pharmaceutical and/or functional food products may significantly prolong, delay or diminish occurrence of these diseases or serve as supplements to conventional drugs. The probiotic L. casei has proved its beneficial effects in improving acquired immunity, decreasing colon inflammation, serum cholesterol and increased blood pressure, improving lactose tolerance, controlling irritable bowel syndrome and decreasing risk of colon cancer. The aim of this study was to evaluate the antiinflammatory properties of L. casei loaded in whey proteinalginate microparticles after oral administration to rats in which TNBS-colitis was induced. TNBS model appeared to show high correlation between the pathohistological, immunological and clinical features of the inflammation in IBDs. Comparing to the positive control, the total damage score and colon weight/body weight ratio decreased when L. casei was administered, with non-significant difference when free and encapsulated cells were administered (23% and 31% for the total damage score, and 5% and. 8% for the colon weight/body weight ratio, respectively). The activity of MPO was also decreased with the probiotic administration and the lowest value was observed when microparticulated probiotic was administered. Macroscopic and histological evaluation confirmed the higher potential of the microencapsulated probiotic to decrease the parameters of inflammation (Figure 1). Visible segments of ulcerations were not observed in the fourth group, while at the histological sections subepithelial polymorph nuclear infiltration was observed with preserved epithelium. Also, in this group, dilated blood vessels in submucosal layer and dilated intestinal glands were observed. In conclusion, the microparticluated L. casei showed high potential to be used as adjuvant therapy in IBD when incorporated in pharmaceutical dosage form or functional food product

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Health biotechnology
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Katarina Smilkov
Date Deposited: 16 Mar 2015 09:55
Last Modified: 16 Mar 2015 09:55

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