Evaluation of non-radioactive lutetium- and yttrium-labeled immunoconjugates of rituximab - a vibrational spectroscopy study

Gjorgieva Ackova, Darinka and Smilkov, Katarina and Janevik-Ivanovska, Emilija and Stafilov, Trajče and Arsova-Sarafinovska, Zorica and Makreski, Petre (2015) Evaluation of non-radioactive lutetium- and yttrium-labeled immunoconjugates of rituximab - a vibrational spectroscopy study. Macedonian Journal of Chemistry and Chemical Engineering, 34 (2). pp. 351-362. ISSN 1857-5552

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Abstract

Fourier Transform Infrared (FT-IR) and Raman Spectroscopy were used to study the molecular structure of the recombinant monoclonal antibody and anti-CD20-conjugates which are intended to be used as anti-cancer therapeutic agents. We characterized the secondary structure of a therapeutic immunoconjugates of rituximab, formulated with three different bifunctional chelating agents (p-SCN-Bn-DOTA, p-SCN-Bn-DTPA, 1B4M-DTPA) and labeled with non-radioactive lutetium and yttrium. The secondary structure content of all three immunoconjugates was assessed to be similar to that of unlabeled antibody. In addition, no significant changes upon lyophilizing procedures were observed. The results demonstrate that amide bands could be used as analytical tool which enables quick and reliable way for screening of protein pharmaceuticals during development of lyophilized formulations.

Item Type: Article
Subjects: Natural sciences > Chemical sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Darinka Gorgieva Ackova
Date Deposited: 04 Feb 2015 13:49
Last Modified: 23 Dec 2015 13:13
URI: http://eprints.ugd.edu.mk/id/eprint/12388

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