Immunohistochemical and histomorphometric analysis of the early phase of wound healing following low intensity level er: YAG LASER assisted pocked debridement

Minovska, Ana (2014) Immunohistochemical and histomorphometric analysis of the early phase of wound healing following low intensity level er: YAG LASER assisted pocked debridement. In: 14th World Congress of Laser Dentistry, 2-4 July 2014, Paris, France.

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Abstract

Introduction The concept of laser assisted no-surgical periodontal treatment, using low-level Er:YAG irradiation, is expected to facilitate or promote the normality of tissue repair, and thereby enhance the sequence of events that take the tissues from their injured to their ‘normal’ state. Aim Since, the excellent ablation of tissue within laser assisted pocket treatment with low intensity Er:YAG irradiation is awaited to promote healing, the present study was conducted to provide analysis of inflammatory cells within initial phase of wound healing following laser debridement versus conventional hand instrumentation. Materials and Methods 15 patients were selected with chronic periodontitis and attachment lost 5 mm on one aspect of the tooth. For the purpose of the study a split-mouth design was performed. The active treatment consists of initial therapy- tooth cleaning with ultrasonic device and creation of condition that enhance oral hygiene (if needed) followed by phase 1 of periodontal therapy -“closed debridement” conventional vs. laser assisted, using energy density of about 178 mJ/ mm2 for soft tissue and 256 mJ/ mm2 for scaling and root planning . Results / Evolution description After 72 hour biopsy was taken from the soft tissue wall of the periodontal pocket and tissue were formalin-fixed and paraffin embedded. A routine hematoxylin-eosin stain were perform using following antibodies: myeloperoxidase, CD68, vimentin and CD34.The findings demonstrate pronounce cellular inflammatory response (p<0,001) following conventionally treated versus laser treated group after 72h, but higher CD34 expression in the laser treated group. Conclusion Our results showed that relatively low postoperative cellular inflammatory response seen in laser treated group can be attributed to the very narrow zone of thermal disruption with minimal invasive instrumentation of pocket;higher CD34 expression can be address to lasers transfer of energy to surrounding tissues in the form of heat able to induce a heat shock response.

Item Type: Conference or Workshop Item (Lecture)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Health sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Ana Minovska
Date Deposited: 04 Feb 2015 08:18
Last Modified: 07 Aug 2015 08:19
URI: http://eprints.ugd.edu.mk/id/eprint/12293

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