Assessment of the Genotoxicity of Heavy Metals in Phaseolus vulgaris L. as a Model Plant system by Random Amplified Polymorphic DNA (RAPD) Analysis

Gjorgieva Ackova, Darinka and Kadifkova-Panovska, Tatjana and Mitrev, Sasa and Kovacevik, Biljana and Arsov, Emilija and Baceva, Katerina and Stafilov, Trajče (2012) Assessment of the Genotoxicity of Heavy Metals in Phaseolus vulgaris L. as a Model Plant system by Random Amplified Polymorphic DNA (RAPD) Analysis. Journal of Environmental Science and Health, Part A, 47. pp. 366-373. ISSN 1093-4529 (Print); 1532-4117 (Online)

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Abstract

Impact assessments of environmental pollutants are important in eco-genotoxicology. A random amplified polymorphic DNA(RAPD) technique was used to detect genotoxicity-induced DNA damage in Phaseolus vulgaris L. from heavy metals at two different concentrations. The results from six 10-base pair (bp) random RAPD primers with 60–70% GC content used, showed a total of 295 RAPD fragments of 700–4000 bp in molecular size in the seedlings of untreated and treated samples, of which only 163 fragments were polymorphic. Polymorphisms became evident as the disappearance and/or appearance of DNA fragments in treated samples compared to the control. A dendrogram constructed using the Numerical Taxonomy and Multivariate Analysis System (NTSYSps), showed that the control group merged with groups treated with CuSO4·5H2O (150 mg L−1) and MnSO4·H2O (150 mg L−1) in a separate cluster. These groups were linked with all of the other samples treated with metals at concentrations of 150 mg L−1 and CuSO4·5H2O and Cd(NO3)2 at concentrations of 350 mg L−1. Finally, the samples treated with metals at concentrations of 350 mg L−1 together with NiSO4 at the concentration of 150 mg L−1, clustered separately. The DNA polymorphism detected by RAPD analysis offered a useful biomarker assay for the detection of toxic chemicals genotoxicity in plant model systems.

Item Type: Article
Subjects: Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Darinka Gorgieva Ackova
Date Deposited: 19 Nov 2012 12:38
Last Modified: 06 Mar 2018 10:38
URI: http://eprints.ugd.edu.mk/id/eprint/1185

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