Development and preclinical evaluations of therapeutic radiopharmaceuticals based on Lu-177 and Y-90 labeled monoclonal antibodies and peptides - Establishment and standardization of a technology for ready to use production of cold kit formulation of DOTA-Rituximab and peptide based radiopharmaceuticals for labeling with lu-177 and Y-90

Janevik-Ivanovska, Emilija and Gjorgieva Ackova, Darinka and Smilkov, Katarina and Stafilov, Trajče and Gjorgoski, Icko (2012) Development and preclinical evaluations of therapeutic radiopharmaceuticals based on Lu-177 and Y-90 labeled monoclonal antibodies and peptides - Establishment and standardization of a technology for ready to use production of cold kit formulation of DOTA-Rituximab and peptide based radiopharmaceuticals for labeling with lu-177 and Y-90. [Project]

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IAEA - CRP project - CRP F22052.pdf

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IAEA CRP F22052- PROJECT RENEWAL 16651R2 - 2014.pdf

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IAEA CRP - F22052 Report - Republic of Macedonia 2013.pdf

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IAEA - CRP F22052 - Report - Republic of Macedonia 2014.pdf

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Official URL: http://www-naweb.iaea.org/napc/iachem/CRPs.html

Abstract

The purpose of this investigation was to introduce a technology for ready to use production of cold kit formulation of DOTA-Rituximab and peptide based radiopharmaceuticals (Substance P) for labeling with lu-177 and Y-90, to establish and to standardize the methods for synthesis and conjugation. In the same time using a model system comprised in both isolated cell cultures and animal model the radiolabeled yield, biological properties and pharmacokinetic behavior of 177Lu-DOTA Rituximab and Supstance P to compare and to determine toxicities and therapeutic efficacies. The work for Substance P has the goal to obtain the peptide available for the labeling with lu-177 was necessary to start from the model already used for labeling with Tc-99m and Re-188. For this purpose we synthesized Substance P modified for Tc-99m and Re-188 labeling and examine the characteristics including biodistribution studies in normal mice (Peptide No.1 - Cys-Cys-SP /PCN for Tc-99m labeling and Peptide No.2 - Cys-Cys-SP /PCN for Re-188 labeling). The other part of our work is to establish the procedure of freeze drying to obtain the final kit formulation for the antibody, ready for labeling. The procedure of freeze drying should provide stable formulation identically available for preclinical investigation as the freshly prepared solution. The freeze dried formulation should have the same immunoreactivity of the conjugated antibody before conjugation and after conjugation in the liquid formulation. For this reason the most important steps in which we were working in our first part of the project was to establish the protocol and to estimat the Freeze-Drying Time for Lyophilized Formulations of Monoclonal Antibody in each phase – Prefreezing, Primary Drying and Secondary Drying.

Item Type: Project
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Biological sciences
Natural sciences > Chemical sciences
Divisions: Faculty of Medical Science
Depositing User: Emilija Janevik
Date Deposited: 09 Dec 2014 10:42
Last Modified: 06 Mar 2018 10:32
URI: http://eprints.ugd.edu.mk/id/eprint/11616

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