Imaging of deep venous thrombosis using radioactive labeled Tirofiban: animal model evaluation

Darkovska-Serafimovska, Marija and Janevik-Ivanovska, Emilija and Ugresic, Nenad and Gjorgoski, Icko (2014) Imaging of deep venous thrombosis using radioactive labeled Tirofiban: animal model evaluation. International Journal of Innovative and Applied Research (IJIAR), 2 (11). pp. 9-19. ISSN 2348-0319

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IMAGINGOF DEEP VENOUS THROMBOSISUSINGRADIOACTIVE LABELED TIROFIBAN - ANIMAL MODEL EVALUATION.pdf

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Abstract

Imaging of acute thrombus, especially the very prevalent condition of acute deep vein thrombosis is usually relied on conventional imaging techniques utilizing either ultrasonography or contrast venography. The former procedure is limited by accuracy and the latter by technical considerations. Recent advances in the understanding of the pathogenesis of acute clot at the molecular level have suggested new opportunities for detection of the acute thrombotic process based on the biomolecular behavior of components of the clotting process including the formed element of the blood, the platelet. Thus, development of radiolabelled small peptide or peptidomimetic ligands that can bind platelets and their specific expressed receptor have been suggested as a new approach to detect clot location and, more essentially, determine the age and morphology of the evolving thrombus. This new approach has focused on the use of a series of radiolabelled platelet GPIIb/IIIa receptor antagonists. Tirofiban N-(butylsulfonyl)- 4-O-(4-(4-piperidyl)-L-tyrosine is a non-peptide tyrosine derivate. The aim of the study was to introduce radioactive labelled tirofiban as a specific imaging agent for acute DVT. The labeling was performed with Technetium-99 in the presence of a stannous reducing agent. The labelled preparation showed a fast blood clearance in the normal rat model (without induced thrombosis). More than 80% of the injected dose was eliminated from the circulation in the first hour after injection. Biodistribution and visualization of the labelled molecule was carried out using an experimental model of thrombosis in the male Wistar rat. Planar images were obtained 30 min and 60 min after application of 2 × 106 imp/min 99mTechnetium-tirofiban, in the rat’s tail vein. Sensitivity and specificity were determined using the ratio ‘left leg positive for DVT’ and ‘right leg negative for DVT’. The obtained ratio was 1.54 after 30 min and 5.04 after 60 min. These values were considered as positive in the detection of acute DVT. The high DVT uptake show that radiolabelled tirofiban in the introduced rat model can be the promising agent for imaging of deep venous thrombosis.

Item Type: Article
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Divisions: Faculty of Medical Science
Depositing User: Emilija Janevik
Date Deposited: 01 Dec 2014 14:13
Last Modified: 01 Dec 2014 14:13
URI: http://eprints.ugd.edu.mk/id/eprint/11555

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