Matrix metalloproteinase activity in deciduous dental pulp

Georgiev, Zlatko and Kovacevska, Ivona and Dimova, Cena (2014) Matrix metalloproteinase activity in deciduous dental pulp. In: 19th Congress of the Balkan Stomatological Society (BaSS), 24-27 Apr 2014, Belgrade, Serbia.

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Abstract

The aim of the present study was to examine the COLLAGENASE activity of the deciduous dental pulp in the normal conditions, and conditions of physiological resorption of the tooth. Decuduous dental pulp was obtained from healthy deciduous teeth, extracted for orthodontic reason. The tooth pulp was excavated completely, washed with sterile 0,08 mol/L NaCl, weighed and frizzed to -18° C. The pulp tissue was transferred to a medium (phosphate buffer, pH = 7,4; 0,08 mol/L NaCl), and homogenized in a Potter-Elvehjem homogenizer. After 10 minutes centrifugation with 3000 rpm and decantation, the enzyme activity was determined in supernatant, Totals pulp enzymatic activity of the deciduous teeth decreases with the beginning of the resorption. Enzymes, which actively participate in metabolism (lactate-dehydrogenase), as well as in synthesis (alcal-phosphatase), can increase the specific activity in conditions of progressive physiological root resorption, because of its discontinuity, in the periods when the reparatory component dominates. Enzymes responsible for tissue destructive changes (acid-phosphatase and collagenase), by initiation and progression of physiological resorption increase significantly.

Item Type: Conference or Workshop Item (Lecture)
Subjects: Medical and Health Sciences > Basic medicine
Medical and Health Sciences > Clinical medicine
Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Ivona Kovacevska
Date Deposited: 20 Nov 2014 11:27
Last Modified: 20 Nov 2014 11:27
URI: http://eprints.ugd.edu.mk/id/eprint/11335

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