Rapid estimation of antioxidant capacity of some medicinal plants: electrochemical and photometric approaches

Naumova, Galaba and Maksimova, Viktorija and Mirceski, Valentin and Ruskovska, Tatjana and Koleva Gudeva, Liljana and Gulaboski, Rubin (2014) Rapid estimation of antioxidant capacity of some medicinal plants: electrochemical and photometric approaches. In: 8th Conference on medicinal and aromatic plants of southeast european countries, 19-22 May 2014, Durres, Albania.

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Abstract

Antioxidants are compounds capable to either delay or inhibit the oxidation processes which occur under the influence of reactive oxygen species. Plant phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, and tannins), nitrogen compounds (alkaloids, amines), carotenoids and vitamins are the most important plant substances presenting antioxidant activity. The total antioxidant capacity (TAC) of some medicinal plants collected in the region of Malesevo Mountain was evaluated using cyclic voltammetry and FRAP method. Infusions samples in the present research were prepared from: Mountain tea (Origanum vulgare L.), Lemon balm (Melissa officinalis L.), St. John’s wort (Hypericum perforatum L.), Wild thyme (Thymus serpyllum L.) and Mint tea (Mentha piperita L.). In this study we present rapid and simple electrochemical voltammetric method for estimation the total antioxidant capacity of medicinal plants in an ethanol/water phase by measuring the rate of homogeneous redox reaction with ABTS•+ radical (2,2’- azinobis(3-ethylbenzothiazoline-6-sulfonic acid)) by means of cyclic voltammetry. ABTS•+ radical was electrochemically in situ generated at the surface of glassy carbon electrode by electrochemical oxidation of ABTS in ethanol electrolyte solution. The method is based on the well-known regenerative catalytic EC´ mechanism, where the ABTS•+ radical serves as a redox mediator for catalytic oxidation of antioxidants present in the plant infusion. The total antioxidant capacity of herb infusions was determined using photometric FRAP method (Ferric reducing antioxidant power) developed by Benzie and Strain. As a conclusion we can say that there were strong correlations between the results obtained with cyclic voltammetry and FRAP method and both methods can be used forevaluation of total antioxidant capacity in medicinal plants infusions.

Item Type: Conference or Workshop Item (Poster)
Uncontrolled Keywords: antioxidants, total antioxidant capacity, medical plants, cyclic voltammetry, FRAP
Subjects: Medical and Health Sciences > Basic medicine
Natural sciences > Chemical sciences
Divisions: Faculty of Medical Science
Depositing User: Viktorija Maksimova
Date Deposited: 08 Oct 2014 13:03
Last Modified: 08 Oct 2014 13:03
URI: http://eprints.ugd.edu.mk/id/eprint/11079

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