Evaluation radiochemical purity of 177lu-labelled Rituximab conjugates using HPLC method

Sterjova, Marija and Smilkov, Katarina and Gjorgieva Ackova, Darinka and Carollo, Angela and Chinol, Marco and Janevik-Ivanovska, Emilija (2014) Evaluation radiochemical purity of 177lu-labelled Rituximab conjugates using HPLC method. In: From Molecules to Functionalised Materials, 05-10 Sept 2014, Ohrid, Macedonia.

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Abstract

In the field of radiolabelled molecules, Rituximab appear as promising molecules for radiopharmaceutical design, because it can target specifically to antigensin non-Hodgkin lymphoma. In our project, Rituximab was conjugated with DTPA-, DOTA- and 1B4Mand prepared in a form of freeze dried kit formulation and labelled with 177Lu used was 565 MBq (in 5 µL) per kit. The reaction mixture was incubated at 380C for 1 hour. The radiochemical purity of the labeled conjugates was determined using SE-HPLC, Column BioSep-SEC-s3000 (300 x7.5 mm; Phenomenex), with flow rate 1ml/min, isocratic elution – eluent 0.1 M phosphate buffer pH 5.8, UV detection at 220 and 280 nm, analysis time ca. 20 min, sample volume: 20μl. To around 10 μl of radiolabelled conjugate 10 μl of 10 mM DTPA solution was added in order to bind non-reacted 177Lu. HPLC analysis was performed 5 min after DTPA addition using UV detection at 220 nm, 280 nm and radiometric detection. 177Lu-Rituximab radioimunoconjugates with high radiolabelling yield and average of radiochemical purity (above 94.7%) and specific activity up to 1.5GBq/mg was obtained. With the obtained results we can conclude that 177Lu- Rituximab radioimunoconjugates can be used for development of the predclinical studies in experimental animal model. Keywords: HPLC, 177Lu-Ab-DTPA, 77Lu-Ab-1B4M, 177Lu-Ab-DOTA

Item Type: Conference or Workshop Item (Poster)
Subjects: Medical and Health Sciences > Other medical sciences
Divisions: Faculty of Medical Science
Depositing User: Marija Arev
Date Deposited: 23 Sep 2014 14:04
Last Modified: 06 Mar 2018 10:30
URI: http://eprints.ugd.edu.mk/id/eprint/11011

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