The effect of some cytokinins on pepper ogranogenesis (Capsicun annuum L.c.v. Kurtovska kapija and zlaten medal) cultured in vitro

Koleva Gudeva, Liljana and Spasenoski, Mirko (2001) The effect of some cytokinins on pepper ogranogenesis (Capsicun annuum L.c.v. Kurtovska kapija and zlaten medal) cultured in vitro. Годишен зборник 2001, Yearbook, 1. pp. 23-26. ISSN 1409-987X

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Abstract

In this pepper are summarized some experimental results concerning the effect of some cytokininns on organogenesis and regeneration of pepper explants (apical buds) cultured in vitro. Two cultivars of pepper (Capsicum annuum L.) were used as experimental material cv. Kurtovska kapija, a red sweet and c.v.Zlaten medal, red sweet type. The bout types are highly popular in the R. of Macedonia, c.v. Kurtovska kapija is extensively grown in the southeaster region, while Zlaten medal is also present in the northeaster part in Macedonia. The meristematic explants were isolated from aseptically grown seedlings, than they were cultivated on MS medium (Murashige and Skoog 1962). The effect of cytokinines KIN, BAP and ZEA applied in medium alone or with combination of auksins IAA and NAA was exanimate. After 40 days we measured the percentage of callus formation, leaf rosettes formation and root formation. Depending on the concentration of cytokines and their combination with auksins, the percentage of callus formation, leaf rosettes formation and root formation, was different for different combinations. The best result for organogenesis and regeneration on the both types of pepper were shown on the mediums with ZEA. For all examined mediums there was noticed a slight difference between sorts Kurtovska kapija and Zlaten medal, on the organogenesis and regeneration effect.

Item Type: Article
Uncontrolled Keywords: organogenesis, shoot culture, ZEA, BAP, KIN, IAA, and NAA.
Subjects: Agricultural Sciences > Agricultural biotechnology
Divisions: Faculty of Agriculture
Depositing User: Liljana Koleva Gudeva
Date Deposited: 16 Nov 2012 14:39
Last Modified: 10 Jun 2013 09:45
URI: http://eprints.ugd.edu.mk/id/eprint/1055

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